Flow Cytometer Sorter - Sony SH800Z

The Sony SH800Z Sorting Flow Cytometer is a compact, easy to use instrument that performs both cell counting and two-way cell sorting. The integrated laser engine houses 4 lasers, providing excitation lines of 405 nm, 488 nm, 561 nm and 638 nm. These lasers are combined and delivered through a single fiber while 6 freeform PMTs allow for the detection of fluorescence signals from any laser based on filter selection.

Manufacturer:? ? ? ? ? ? ? ??Sony
Model No.:? ? ? ? ? ? ? ? ? ? ? ??SH-800Z
Year of manufacture:??2016
Year acquired:? ? ? ? ? ? ? ??2016
Location:? ? ? ? ? ? ? ? ? ? ? ? ??Parsons Room W118
Specifications:

• Optics
  • Excitation Lasers: 405nm, 488nm, 561nm, 638nm
  • Output Power: 30mW (max.) optical fiber output
  • Beam Alignment:? Collinear optical system
  • Detection Parameters: 6 Fluorescent + 2 Scatter
  • Pulse Measurement:? Height, area, width
• Fluidics
  • Single, auto-loading tube
  • Sample Tube Types:? 0.5ml, 1.5ml, 5 ml, 15 ml tubes
  • Sort Streams: 2
  • Sort Collection Devices:? 2-way tube (5ml, 15ml), multiwell plates, PCR tubes, slides
  • Temperature Control:? Pre-sort -- 5 ¡ãC, 37 ¡ãC, Post-sort -- 5 ¡ãC
  • Nozzle Sizes: 70, 100, 130 ?m
• Performance
  • Event Rate: 100,000 events per second max
  • Sorting Speed:? Using the 70 ?m sorting chip at 50kHz, an average threshold of 12,000 events per second can be achieved with >95% purity and >80% yield.? The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson's statistics.? Using 100 ?m or 130 ?m sorting chips, maximum sorting speed is 10,000 events per second.
  • Index Sorting: Yes
  • Scatter Resolution: 0.5 ?m
  • Fluorescense Sensitivity: FITC 120 MESF, PE 110 MESF

Principal?scientist: Mark Townley

188ÌåÓýapp_188ÌåÓýÔÚÏß-ƽ̨¹ÙÍø UIC KNOWLEDGE BASE

The Knowledge Base contains forms, instruction?and training material, minutes, policies, tools and other resources to support your research efforts by topic area.

• UIC: Online Instrument Reservation
• UIC: Flow Cytometer FAQs
• UIC: Training Form
• UIC: Sample Submission Form
• March 21, 2023: Flow Cytometer Workshop Materials

W123 Parsons Hall
Durham, NH 03824
Phone: (603) 862-2790

UNH Rate:?
$59.00/hour? assisted
$11.00/hour? unassisted

Non UNH academic Rate:?
$118.00/hour? assisted
? $22.00/hour? unassisted

Industry Rate:
$150.00/hour? assisted
$100.00/hour? unassisted