The Becton-Dickinson FACSCalibur Flow Cytometer is a four-color, dual-laser, bench-top system capable of cell analysis using forward scatter, side scatter, and detection of fluorescence in four distinct color regions: > 670 nm (deep red), 653-669 nm (red), 564-606 nm (orange), and 515-545 nm (green). The unit has a Mac G4 host computer, and most instrument functions are computer controlled.
The unit has two lasers for exciting fluorochromes: an argon laser, which emits sapphire-colored light at 488 nm, and a red diode laser emitting light at 635 nm. This unit is best suited for the analysis of aqueous suspensions of cells or particles with diameters between 1 and 50 um (microns). Ideally samples should contain 500,000 cells or particles per mL. Sample consumption can be varied between 12 uL/min and 60 uL/min, and so small samples can be analyzed relatively easily. All samples to be run should be non-toxic (given the present instrument siting) and be completely fixed (cells should be dead).
Manufacturer:? ? ? ? ? ? ? ? Becton-Dickinson
Model No.:? ? ? ? ? ? ? ? ? ? ? ? Facs-Calibur
Year of manufacture:??2000
Year acquired:? ? ? ? ? ? ? ? 2002
Location:? ? ? ? ? ? ? ? ? ? ? ? ? ?Parsons W118
Specifications:? ? ? ? ? ? ? ?Fluorophores are excited at 488 nm or 635 nm with subsequent emitted fluorescence collected at various wavelengths
Principal?scientist: Mark Townley
The Knowledge Base contains forms, instruction?and training material, minutes, policies, tools and other resources to support your research efforts by topic area.
- UIC: Online Instrument Reservation
- UIC: Small Instruments Brochure
- UIC: Flow Cytometer FAQs
- UIC: Training Form
- UIC: Sample Submission Form
Parsons Hall
Durham, NH 03824
Phone:(603) 862-2790
UNH Rate:?
$57.00/hour? assisted
?$10.00/hour? ?unassisted
Non UNH academic Rate:?
$114.00/hour? assisted
?$20.00/hour? ?unassisted
Industry Rate:
$150.00/hour? assisted
$100.00/hour? unassisted
